Table I. Measured values at 25°C, modeled values at 25°C (k25), and Ea using the equation parameter = k25exp(Ea(Tk − 298.15)/(298.15RTk)) for all measured temperature responses ± se.
For parameters kCA and Vpmax, a deactivation was included using the model parameter = k25exp(Ea(Tk − 298.15)/(298.15RTk))(1 + exp((298.15ΔS − Hd)/(298.15R)))/(1 + exp((TkΔS − Hd)/(TkR))), where Tk is the temperature in Kelvin, R is the molar gas constant, ΔS is the entropy factor, and Hd is the heat of deactivation.
| Parameter | Units | Measured at 25°C | k25 | Ea | ΔS | Hd |
|---|---|---|---|---|---|---|
| kJ mol−1 | kJ mol−1 K−1 | kJ mol−1 | ||||
| kCA | μmol CO2 m−2 s−1 Pa−1 | 124 ± 6 | ||||
| Normalized to 1 at 25°C | 1.0 ± 0.02 | 1.03 ± 0.05 | 40.9 ± 70.7 | 0.21 ± 0.19 | 64.5 ± 50.9 | |
| kh | s−1 | 0.039 ± 0.000 | 0.038 ± 0.000 | 95.0 ± 1.0 | – | – |
| Vpmax | μmol HCO3− m−2 s−1 | 450 ± 16 | ||||
| Normalized to 1 at 25°C | 1 | 1.01 ± 0.07 | 94.8 ± 40.8 | 0.25 ± 0.12 | 73.3 ± 39.6 | |
| KP | Pa CO2 | 16.0 ± 1.3 | 13.9 ± 1.0 | 36.3 ± 2.4 | – | – |
| μm HCO3− | 62.8 ± 5.0 | 60.5 ± 2.4 | 27.2 ± 2.8 | – | – | |
| kcatCO2 | mol CO2 mol−1 site s−1 | 5.44 ± 0.44 | ||||
| Vcmax | Normalized to 1 at 25°C | 0.96 ± 0.04 | 0.89 ± 0.05 | 78.0 ± 4.1 | – | – |
| Vomax/Vcmax at 25°C | 0.18 ± 0.03 | 0.16 ± 0.01 | 55.3 ± 6.2 | – | – | |
| KC | Pa of CO2 | 94.7 ± 15.1 | 121 ± 7 | 64.2 ± 4.5 | – | – |
| KO | kPa of oxygen | 28.9 ± 5.4 | 29.2 ± 1.9 | 10.5 ± 4.8 | – | – |
| SC/O | Pa Pa−1 | 1,610 ± 66 | 1,310 ± 52 | −31.1 ± 2.9 | – | – |