Figure 11.
MYB112 regulates anthocyanin accumulation under high light. A, MYB112 promoter activity in 10-d-old Arabidopsis ProMYB112:GUS seedlings (lines 2 and 7) treated for 6 h with high light (500 µE; right) compared with control seedlings kept at 100 µE (left). B, GUS activity in 3-week-old ProMYB112:GUS plants treated with high light for 20 h (right) compared with control plants kept at 100 µE (left). Staining in A and B is shown for two lines (i.e. GUS#2 and GUS#7). Note the enhanced GUS staining of plants treated with high light. C, Anthocyanin content in myb112 mutants grown under high light. Plants were grown in long-day condition and after 3 weeks transferred to high light (500 µE) for 3 d. Total anthocyanin content was measured spectrophotometrically after extraction with HCl solution. Means ± sd are shown for five biological replicates. FW, Frozen weight. *, Statistically significant difference to wild-type (Wt) plants as determined by Student’s t test (P < 0.05).