Figure 5.

MYB112 expression is affected by neither Suc nor nitrogen. A, Transcript levels of PAP1 and MYB112 determined by qRT-PCR in 2-week-old seedlings grown on MS with 1% (control) or 4% (w/v) Suc. Note the absence of an effect of elevated Suc concentration on MYB112 expression. The data are represented as fold change compared with the expression in control seedlings. Means of three biological replicates ± sd are shown. *, Statistically significant difference compared with control plants as determined by Student’s t test (P < 0.01). B, Expression level of PAP1 and MYB112 in plants grown on medium with or without nitrogen. Plants were grown in a hydroponic system and after 19 d transferred to medium without a nitrogen source. Gene expression was measured 7 d after the transfer. MYB112 expression is not affected by nitrogen availability, whereas PAP1 expression increases by approximately 8-fold. Data were extracted from Affymetrix ATH1 hybridizations (Balazadeh et al., 2014) and represent the means ± sds of three biological replicates. Numbers indicate log₂ values. *, Statistically significant difference compared with control plants as determined by Student’s t test (P < 0.05).