Figure 7.
MYB112 transactivates MYB7, MYB32, and MYB6 promoters. Transactivation of MYB7 (A), MYB32 (B), and MYB6 (C) expression by MYB112 in Arabidopsis mesophyll cell protoplasts. D, Absence of transactivation of the CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE18 (CIPK18) promoter (negative control [Neg]). The ProMYB7:fLUC, ProMYB32:fLUC, ProMYB6:fLUC, or ProCIPK18:fLUC constructs harboring the respective promoters (approximately 1.7 kb) upstream of the fLUC open reading frame were cotransformed with the 35S:MYB112 plasmid (omitted in control experiments). The 35S:rLUC vector was used for transformation efficiency normalization. Given are means ± sd of four biological replicates. *, Significant differences to control as determined by Student’s t test (P < 0.05).