HCT1806 and HCT4918 suppressed Rp1-D21-induced HR. A, The positions of genes (HCT1806, HCT4918, AAS6469, and AAS3125) relative to the SNP on chromosome 1 were shown to be highly associated with variation in Rp1-D21-induced HR from a previous study (Olukolu et al., 2014). Arrows indicate the direction of transcription of the genes. B, The phenotypes resulting from transient coexpression of HCT1806, HCT4918, AAS6469, AAS3125, or Gus with Rp1-D21. The pictures were taken at 3 d after inoculation. The two pictures show the same leaf before (left) and after (right) ethanol clearing. C, Ion leakage conductivity was measured at 60 h after coexpression of HCT1806, HCT4918, AAS6469, AAS3125, or Gus with Rp1-D21. Significant differences (P < 0.05) between samples are indicated by different letters (a and b). D, Total protein was extracted from agro-infiltrated leaves at 30 h post inoculation (hpi). Anti-HA was used to detect the expression of Rp1-D21, and anti-GFP was used to detect HCT1806, HCT4918, AAS6469, AAS3125, or Gus. Equal loading of protein samples is shown by Ponceau S staining. Each experiment was performed three times with similar results.