Figure 3.

Investigating the function of maize HCT homologs in Rp1-D21-induced HR. A, The up-regulated HCT homologs were transiently coexpressed with Rp1-D21 into N. benthamiana. The representative leaves were photographed at 3 d after inoculation (upper), and the same leaves were cleared by ethanol (lower). B, Ion leakage conductivity was measured at 60 h after coexpression of Gus or HCT homologs with Rp1-D21. Significant differences (P < 0.05) between samples are indicated by different letters (a–c). C, Total protein was extracted from agro-infiltrated leaves at 30 hpi. Anti-HA was used to detect the expression of Rp1-D21, and anti-GFP was used to detect the expression of Gus or HCT homologs. Equal loading of protein samples is shown by Ponceau S staining. The experiments were repeated three times with similar results.