Figure 6.
Investigating the interactions between Rp1 proteins and HCT1806/HCT4918/HCT7251 by BiFC assay in N. benthamiana. Rp1-D21/Rp1-D/Rp1-dp2 was fused with cEYFP, and HCT homologs were fused with nEYFP vectors. The cEYFP- and nEYFP-derived constructs were transiently agro-infiltrated into N. benthamiana. The pictures were taken at 40 h postinfiltration under a confocal microscope. Green fluorescence indicates interaction between constructs tagged with nEYFP and constructs tagged with cEYFP. The experiments were repeated three times with the similar results. Bar = 20 µm.