Figure 1.

Growth and starch staining of be2be3 mutants expressing different BE types. The class II BE from maize (ZmBE2a), the class I BE from potato (StBE1), and the glycogen BE from E. coli (EcGLGB) were expressed in the Arabidopsis be2be3 double mutant. For each transformation, three independent lines are shown (designated 1–3 in each case). A, Thirty days after germination, plants were harvested at the end of the day, weighed, and photographed. Representative plants for each transgenic line are shown. The rosette fresh weight for each line is given (mean ± sem of between four and eight plants). Significant differences in growth rate, as determined by ANOVA analysis (P < 0.05), are designated by the letters a and b. B, Plants, like in A, were decolorized in hot ethanol and stained for starch with iodine-potassium iodide solution. After rinsing in water, the plants were photographed. Note that the be2be3 parental line does not stain and the differences in starch staining between the lines transformed with different genes.