Table I. Quantification of BE activity in plants expressing different BE types.
For each sample, six leaves from six plants of each line were harvested in the middle of day and pooled. Extracts of soluble proteins (10 µg) were added to an assay mixture containing 50 mm Glc-1-P, including [U-14C]Glc-1-P. The reactions were started by adding 2 units of phosphorylase-a, and they were incubated at 25°C for 3 h. The activity of the BE from each line was calculated from the rate obtained between 60 and 180 min (mean ± se; n = 3).
| Genotype | BE Activity |
|---|---|
| nmol Glc-1-P incorporation min−1 mg−1 protein | |
| Wild type (WS) | 476 ± 47 |
| be2be3 | 27 ± 50 |
| ZmBE2a-1 | 1,740 ± 6 |
| ZmBE2a-2 | 1,591 ± 79 |
| ZmBE2a-3 | 318 ± 24 |
| StBE1-1 | 230 ± 39 |
| StBE1-2 | 232 ± 32 |
| StBE1-3 | 54 ± 15 |
| EcGLGB-1 | 344 ± 39 |
| EcGLGB-2 | 223 ± 14 |
| EcGLGB-3 | 216 ± 56 |