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. 2015 Jul 13;169(3):1656–1670. doi: 10.1104/pp.15.00794

Figure 5.

Figure 5.

APGs decrease in both transient and stable CHS-silenced fruits. Fragaria spp. fruit phenotypes and CHS gene expression levels in agroinfiltrated fruits (A), and effect of CHS gene down-regulation on APGs (M1–M3) in transiently CHS-silenced fruits (B; CHSi, strawberry ‘Mara des Bois’) and a stable CHS antisense (antis.) transgenic line (C; CHS antis.; strawberry ‘Calypso’). Fourteen days after pollination, green Fragaria spp. fruit was infiltrated with Agrobacterium tumefaciens transformed with a construct encoding CHSi. Levels of metabolites were determined by LC-MS 14 d after infiltration in CHS-silenced fruits (CHSi; n = 5) and fruits infiltrated with A. tumefaciens containing the control vector (PBI, pBI-Intron; n = 5). Down-regulation of the CHS genes results in a decrease of APGs in the CHSi fruits and fruits of the stable transgenic line (CHS antis.; strawberry ‘Calypso’) compared with the control fruits (pBI-Intron and the wild type; for each, n = 5). Relative concentration (rel. conc.) is expressed in milligram equivalent internal standard g–1. Asterisks indicate significant differences (*P = 0.01).