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. 2015 Jul 13;169(3):1656–1670. doi: 10.1104/pp.15.00794

Table IV. Detection of isotopically labeled APGs.

Isotopically labeled APGs were detected 1 d after injection of different concentrations of l-Ile-13C6, l-Leu-13C6, and l-Val-2,3,4,4,4,5,5,5-D8 into strawberry ‘Mara des Bois’ fruits.

Concentration Applied Substrates Compound Products
Unlabeled Labeled
mm %
10 l-Leu M3 100a 0b
50 l-Leu M3 100 0
10 l-Leu-13C6 M3 100 19.7 ± 1.3
50 l-Leu-13C6 M3 100 78.7 ± 6.3
10 l-Ile M1 100 0
50 l-Ile M1 100 0
10 l-Ile-13C6 M1 100 15.9 ± 5.3
50 l-Ile-13C6 M1 100 40.4 ± 0.3
10 l-Val M2 100 0
50 l-Val M2 100 0
10 l-Val-2,3,4,4,4,5,5,5-D8 M2 100 22.9 ± 4.7
50 l-Val-2,3,4,4,4,5,5,5-D8 M2 100 48.0 ± 24.9
a

Integrated peak areas of pseudomolecular ions m/z –371, –371, and –357 (after application of l-Leu, l-Ile, and l-Val, respectively) were set to 100%.  b Percentages of labeled products were calculated from the integrated peak areas of the pseudomolecular ions of isotopically labeled products m/z 376, –376, and –364 (after application of l-Leu-13C6, l-Ile-13C6, and l-Val-2,3,4,4,4,5,5,5-D8, respectively).