Formation of callose deposits in roots of wild-type (wt) and mutant plants after 9-HOT treatment. A, Aniline Blue staining of wild-type plants grown on MS alone or treated with BZ (1 μm) or BL (10 nm). B, Fluorescence of callose deposits by Aniline Blue staining in 9-HOT-treated roots (25 μm) of wild-type, bri1-1, det2-1, and noxy2-2 plants. The bottom row shows callose deposition in roots treated in addition with BZ (1 μm) or BL (10 nm). C, bes1-D and brz1-1D plants grown on MS or treated with 9-HOT. Bars = 100 μm.