Overview of gene expression clusters calculated by K-means clustering. A, Pearson correlation was used to identify six clusters involving a total of 1,607 transcripts with significant expression profile changes for at least one time point after the initiation of aphid feeding. The total number of transcripts in each cluster is indicated, and data for individual genes are shown in light gray. Average expression responses for each cluster are shown in red. All genes selected for this analysis have significant differences of 2-fold (up- or down-regulated), P < 0.05 (FDR adjusted). B, Overrepresentation analysis of each cluster using the MetGenMAP Web site to identify metabolic functions that are being regulated.