Oxylipins produced in xylem contact cells activate JAZ10 expression throughout the vascular cylinder and in extraveinal cells. A and B, Cross sections through the midvein showing the localization of LOX6-GUS fusion protein expressed under the LOX6 promoter before (A) and after (B) wounding. C and D, JAZ10p:GUS activity in the wild type (WT) before (C) and after (D) wounding. E and F, JAZ10p:GUS activity in the lox234 triple mutant before (E) and after (F) wounding. L4 of 21-d-old plants was stained for GUS activity in control samples and 6 h after wounding L3. c, Xylem contact cell; m, mesophyll; p, phloem region including phloem parenchyma; v, xylem vessel. Bar = 50 μm.