Figure 5.

Gene expression analysis for OsEREBP1 (LOC_Os02g54160) and OsEREBP2 (LOC_Os01g64790) was performed by RT-qPCR in wild-type (WT) and RNAi::OsHOS1 silencing lines (#1 and #2–4) under control conditions. The specific primers are described in Supplemental Table S2. The transcript level for OsUBC2 (LOC_Os02g42314) was used as an internal control for the RT-qPCR. The expression value of the wild-type sample was set to 1. Values represent means ± se (n ≥ 5 biological samples from at least three independent assays). No significant statistical differences were observed within the groups using ANOVA one-way analysis (P < 0.05).