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. 2015 Sep 17;169(3):2275–2287. doi: 10.1104/pp.15.01131

Figure 7.

Figure 7.

Proteasome-mediated degradation of GST-OsEREBP1 and GST-OsEREBP2. Crude protein extracts from RNAi::OsHOS1#1 and wild-type (WT) plants under control conditions were incubated at 30°C with GST-OsEREBP1 or GST-OsEREBP2, with or without MG132, for the different sampling time points shown. Control represents the GST-OsEREBP1 (or GST-OsEREBP2) input for all assays without crude protein extract at time 0 or after 120 min of incubation at 30°C. The different samples were resolved on 10% (v/v) SDS-PAGE, and the detection of GST-tagged proteins was made as described in “Materials and Methods.” The intensity of Coomassie Blue (CBB) staining was used as a loading control of the protein extracts from RNAi::OsHOS1#1 and wild-type plants. The immunoblot results are representative of two technical replicates from two independent biological replicates. The immunoblot bands (at time 120 min) were quantified using ImageJ software and normalized against Coomassie Blue total protein. Values represent means ± se.