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Fluorescence lifetimes in FRET-FLIM interactions. The bar graphs represent average fluorescence lifetimes (ns) and the corresponding sd values for the GFP donors RTNLB3 and RTNLB6. The data show 17 candidate interaction proteins (blue bars) compared with RTNLB3-GFP or RTNLB6-GFP without interaction partners (gray bars). Lifetimes significantly lower than those of RTNLB3-GFP or RTNLB6-GFP alone (left side of the red line) indicate protein-protein interactions.
