Figure 2.
Allelic variants of qSD1-2. A, Gene structure. SD1-2S, SD1-2E, and SD1-2D are alleles from SS18-2, EM93-1, and cv Dongjin, respectively. Boxes indicate 5′/3′ UTRs (white) or exons (Ex.; black), and line segments indicate introns. Vertical lines indicate positions of point mutations as compared with SD1-2S. The gap in SD1-2E represents a deletion resulting in a premature stop codon at the arrow-pointed position. B, cDNAs. The scale indicates lengths of UTRs (white boxes) and exons (gray boxes). Arrows indicate positions of PCR primers used to amplify the overlapped fragments on the gel image. C, Protein sequences. Amino acid sequences were deduced based on the cDNA sequences for SD1-2S and SD1-2E or on the genomic DNA sequence for SD1-2D. Underlined sequences are predicted functional domains, asterisks indicate residues different between SD1-2S and SD1-2D, and SD1-2E is a truncated protein.