Figure 4.

Ectopic expression of BdWRI1 increases TAG content in B. distachyon leaf blades. A and B, Expression levels of BdWRI1 (A) glycolysis and FA biosynthesis orthologs (B) in 8-week-old leaf blades. Total RNA was isolated, and relative mRNA levels were determined by quantitative RT-PCR using BdUBC18 as an internal control. Three independent measurements were averaged, and the error bars represent sd. Asterisks indicate significant differences by Student’s t test: *, P < 0.05; and **, P ≤ 0.01. C and D, TAG contents (C) and FA composition of TAG (D) of 8-week-old leaf blades. Total lipids were extracted, and TAG was separated by thin-layer chromatography (TLC). Three independent measurements were averaged, and the error bars represent sd. Asterisks indicate significant differences by Student’s t test: *, P < 0.05; and **, P ≤ 0.01. DW, Dry weight; WT, wild type.