Table II. Transcriptional changes of hyperosmotic stress-inducible genes in the ∆4 mutant under HC conditions.

This table features a list of hyperosmotic stress-inducible genes identified by Paithoonrangsarid et al. (2004) and the respective transcriptional changes in the ∆4 mutant under HC conditions. Fold changes in expression levels were regarded as significant (boldface) if log₂ of the change in expression level was −1 or less or +1 or more and at P ≤ 0.05. Transcripts showing an inverse regulation in the wild type and the ∆4 mutant are underlined. All transcript data from these analyses were normalized to the paired average expression levels of the wild type under HC conditions. All transcript data were averaged from two independently repeated biological shift experiments per genotype. Groups are according to Paithoonrangsarid et al. (2004): group 1, genes in which induction by hyperosmotic stress was diminished or significantly reduced in Δhik33 cells; group 2, genes in which induction by hyperosmotic stress was diminished or significantly reduced in Δhik34 cells; group 3, genes in which induction by hyperosmotic stress was diminished or significantly reduced in Δhik16 and Δhik41 cells; group 4, genes in which induction by hyperosmotic stress was unaffected in Δhik33, Δhik34, Δhik16, or Δhik41 cells.

Systematic Gene Name	Gene Name	Protein Name	Wild-Type 24-h LC Conditions/Wild-Type HC Conditions	∆4 24-h HC Conditions/Wild-Type HC Conditions
Group 1
sll1483		Periplasmic protein, similar to transforming growth factor-induced protein	−0.43	3.22
sll0330	fabG	Sepiapterin reductase	0.23	3.99
slr1544		Unknown protein	−1.13	2.90
ssl2542	hliA, scpC	High-light-inducible polypeptide HliA, CAB/ELIP /HLIP superfamily	−0.64	0.39
ssr2595	hliB, scpD	High-light-inducible polypeptide HliB, CAB/ELIP /HLIP superfamily	−0.96	2.73
ssr2016		Hypothetical protein	−0.61	3.13
ssl1633	hliC, scpB	High-light-inducible polypeptide HliC, CAB/ELIP /HLIP superfamily	0.19	1.57
ssl3446		Hypothetical protein	0.01	3.31
slr0381		Lactoylglutathione lyase	0.30	0.00
sll2012	sigD	Group 2 RNA polymerase sigma factor SigD	1.30	1.99
sll1541		Hypothetical protein	0.02	0.43
Group 2
sll1514	hspA	16.6-kD small heat shock protein, molecular chaperone	−0.94	3.09
sll0846		Hypothetical protein	−0.35	3.18
slr1963	ocp	Water-soluble carotenoid protein	−0.88	0.99
slr1641	clpB1	ClpB protein	−0.07	1.88
slr0959		Hypothetical protein	−0.19	1.92
slr1516	sodB	Superoxide dismutase	0.01	1.29
sll0430	htpG	HtpG, heat shock protein90, molecular chaperone	−0.26	0.79
sll1884		Hypothetical protein	−0.07	0.93
slr1603		Hypothetical protein	0.54	1.75
slr1915		Hypothetical protein	0.05	2.42
ssl2971		Hypothetical protein	−0.28	0.17
slr1285	hik34	Two-component sensor His kinase	−0.23	2.37
slr1413		Hypothetical protein	0.11	1.04
sll0170	dnaK2	DnaK protein2, heat shock protein70, molecular chaperone	0.16	2.21
sll0005		Hypothetical protein	0.65	0.49
slr2076	groEL1	60-kD chaperonin	−1.29	1.11
slr0093	dnaJ	DnaJ protein, heat shock protein40, molecular chaperone	0.04	1.51
slr2075	groES	10-kD chaperonin	−1.60	1.88
sll0416	groEL2	60-kD chaperonin2, GroEL2, molecular chaperone	−1.45	1.66
Group 3
sll0939		Hypothetical protein	−0.09	−0.17
slr0967		Hypothetical protein	−1.20	3.30
Group 4
sll1863		Unknown protein	−1.13	0.76
sll1862		Unknown protein	−0.24	1.08
sll0528		Hypothetical protein	−0.13	3.06
slr1204	htrA	Protease	−0.96	3.40
slr0423	rlpA	Hypothetical protein	0.21	0.64
sll1722		Hypothetical protein	0.08	1.75
sll0306	sigB	RNA polymerase group 2 sigma factor	0.62	2.99
slr0581		Unknown protein	0.32	2.53
ssr1853		Unknown protein	0.35	0.62
slr1119		Hypothetical protein	0.31	0.55
slr0852		Hypothetical protein	0.22	2.42
ssr3188		Hypothetical protein	0.48	0.51
slr0112		Unknown protein	0.41	1.42
sll0294		Hypothetical protein	0.09	0.75
slr0895		Transcriptional regulator	−0.56	−0.48
ssl3177	repA	Hypothetical protein	−0.37	1.48
sll1085	glpD	Glycerol-3-phosphate dehydrogenase	0.33	0.32
slr1051	envM	Enoyl-[acyl carrier protein] reductase	−0.63	−0.33
sll0293		Unknown protein	0.46	1.21
sll0470		Hypothetical protein	−0.17	1.42