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. 2015 Oct 15;6:8549. doi: 10.1038/ncomms9549

Figure 4. RFX3 expression in the mouse inner ear.

Figure 4

Inner ear sections from a P1 wild-type mouse stained with an antibody for MYO6, which marks the inner ear hair cells (left panel) and RFX3 (right panel), in red, and counterstained with DAPI, in blue. A robust nuclear expression of RFX3 is detected in all HCs with a much weaker expression in ENHCs. Staining of the stereocilia with the RFX3 antibody is non-specific, validated by staining cKO ears in which the nuclear staining is abolished and the stereocilia staining persists (Supplementary Fig. 11). IHC, inner hair cell; OHCs, outer hair cells. Representative images of n>5 experiments. Scale bar, 60 μm.