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. 2015 Nov 4;15:256. doi: 10.1186/s12866-015-0586-2

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© Assato et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Evaluation of complementation. A spot assay was used to evaluate susceptibility to fluconazole 35 μM in wt (a), Δbmh1 (b) and Δbmh2 (c) S. cerevisiae transformants. There was a decreased sensitivity of transformants pYES-14-3-3 and pYES-BMH1 compared with the empty vector transformant (pYES). As a growth control, the transformants were also spotted in SD-URA without fluconazole