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. 2015 Oct 29;11(10):e1005220. doi: 10.1371/journal.ppat.1005220

Fig 3. STIM1 suppression inhibits egress of eVP40 VLPs.

Fig 3

A. VP40 was expressed in control and STIM1 suppressed HEK293T cells as indicated and eVP40 VLP production quantified by immunoblot analysis demonstrates significant inhibition of eVP40 production in STIM1 suppressed cells (n = 4 independent experiments). B. Immunoblot analysis of cell extracts and VLPs from HEK293T cells expressing eVP40 in cells co-transfected with indicated amounts of control vector (top panel), STIM1 suppression plasmid (middle panel), or shSTIM1-STIM1 suppression-rescue plasmid (bottom panel). C. Cellular VP40 and STIM1 levels were measured in mock-transfected HEK293T cells, or cells transfected with the indicated plasmids and siRNAs. Results are representative of three independent experiments.