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. 2013 Sep 6;7(6):1210–1217. doi: 10.1111/irv.12150

Figure 1.

Figure 1

Intramuscular inoculation of inactivated PR8 influenza virus (10 HAU/mouse) induced the highest production of anti‐PR8 IgG2a and upregulated TLR7 expression. (A, B) Mice were inoculated with inactivated PR8 influenza virus on days 0 and 7 by different routes. (A) Serum anti‐PR8 IgG2a was measured on days 0, 7 and 14 by ELISA. (B) BAL total cells were counted. i.m., intramuscular; i.n., intranasal; s.c. subcutaneous. N = 4–5 mice per group. (C) Mice were received intramuscular injection of PBS or inactivated PR8 influenza virus. After 18 hours, the muscles near the site of injection were isolated and expression of TLR‐7 was detected by quantitative RT‐PCR. Relative mRNA was calculated by normalizing the values of the TLR7 to that of β‐actin. *P < 0·05; ***P < 0·005.