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. 2015 Sep 11;24(23):6588–6602. doi: 10.1093/hmg/ddv363

Figure 2.

Figure 2.

LGMD1D mutant DNAJB6b is more stable than DNAJB6b-WT in vivo. (A) Immunoblot of skeletal muscle lysates from control (C57), V5-hDNAJB6b-WT (b-WT), V5-hDNAJB6b-F93L (b-F93L), V5-hDNAJB6a-WT (a-WT) and V5-hDNAJB6a-F93L (a-F93L) expressing mice using a V5 antibody. Actin and GAPDH are loading controls. (B) Quantitative PCR expression matched V5-hDNAJB6b-WT and V5-hDNAJB6b-F93L lines and corresponding V5 immunoblot from the same lysate. Note that hDNAJBb-F93L is more stable. (C) Tetracycline inducible isogenic 293 cell lines expressing V5-DNAJB6b-WT or V5-DNAJB6b-F93L were induced for 24 h and then tetracycline was removed and lysates were immunoblotted for V5 and GAPDH at the indicated times. (D) Graphical representation of the results in C from three independent experiments.