Figure 1.

miR-132/212 deficiency alters tau expression and phosphorylation in mice. (A, B, C) Representative western blot of endogenous tau and phospho-tau in 6-month-old miR-132/212 KO mice and wild-type littermate controls (n = 8/group, cortex, mixed gender). Total tau or Gapdh was used as normalization control, and quantifications are shown. (D) Real-time quantitative RT-PCR of endogenous tau mRNA in WT and KO mice aged 6 months (n = 5/group, mixed gender). Gapdh mRNA was used as normalization control. (E, F, G) Representative western blot of endogenous GSK-3β, phospho-GSK-3β S9 (inactivated form) and PP2B (n = 8/group, cortex, mixed gender). Gapdh was used as normalization control, and relative quantifications are shown. Statistical significance was determined by Student's unpaired t-test, where *P < 0.05 and ***P < 0.001