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. 2015 Sep 16;24(23):6769–6787. doi: 10.1093/hmg/ddv382

Figure 5.

Figure 5.

TGFBRI, TGFBRII and CTGF expression in ATS fibroblasts. (A) WB of 50 µg cell membrane-bound proteins extracted from control (C1 and C2) and ATS (P1, P2 and P3) fibroblasts immunoreacted with the anti-human TGFBRI and TGFBRII Abs, detecting a 52 and 70 kDa band, respectively. To analyse the expression of CTGF, which was indicated through a 37 kDa band, the total extract from both cell types was used. Loading control: β-actin. (B) To detect the distribution of the membrane-associated insoluble fraction of CTGF, the control and ATS fibroblasts were fixed and immunoreacted as previously described (34). Scale bar: 4 µm. The experiments were repeated three times. A representative image of two control (C1 and C2) and the three ATS fibroblasts (P1, P2 and P3) is shown.