Table 1.
In vitro resistance to amantadine
| Sample | Date | Country | Type/ Subtype | Substitution conferring resistance | Predicted sensitivity | Predicted IC50(μg amantadine/ml) |
|---|---|---|---|---|---|---|
| FLU5148 | 23‐November‐2006 | Ecuador | A/H1N1 | Leu26Phe | R | >1000 |
| FLU4129 | 01‐August‐2006 | Peru | A/H1N1 | – | S | 0·25 |
| FLU5376 | 31‐January‐2007 | Peru | A/ H1N1 | – | S | 0·3 |
| FLU4499 | 13‐October‐2006 | Nicaragua | A/H1N1 | – | S | 0·3 |
| FLU3443 | 18‐April‐2006 | Peru | A/H3N2 | Ser31Asn | R | >1000 |
| FLU3601 | 31‐March‐2006 | Peru | A/H3N2 | Ser31Asn | R | >1000 |
| FLU6219 | 20‐March‐2007 | Venezuela | A/H3/N2 | Ser31Asn | R | >1000 |
| FLU6849 | 09‐May‐2007 | Ecuador | A/H3N2 | Ser31Asn | R | 1000 |
| lQE5463 | 14‐May‐2007 | Peru | A/H3N2 | Ser31Asn | R | >1000 |
| FLU5674 | 06‐March‐2007 | Peru | A/H3N2 | – | S | 0·25 |
| FLU5854 | 08‐March‐2007 | Peru | A/H3N2 | – | S | 0·5 |
| FSC0799 | 30‐July‐2005 | Peru | A/H3N2 | – | S | 0·2 |
| FLU6151 | 17‐April‐2007 | Peru | B | – | R | >1000 |
The table shows the concentration of amantadine giving 50% of plaque assay inhibition (IC50) for different viruses types and subtypes. The table shows the date and country where the samples were collected, the type and subtype determined based on sequence analysis of RT‐PCR amplicons of the corresponding hemagglutinin and neuraminidase genes, the substitution conferring resistance to amantadine, the inferred sensibility to amantadine and finally their sensibility to amantadine found in vitro.