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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Cell. 2014 Nov 20;159(5):1027–1041. doi: 10.1016/j.cell.2014.10.023

Figure 7.

Figure 7

ER structure and dynamics affect endosome fission. (A) Live Cos-7cells expressing BFP-KDEL and mCh-Rab7 with (top panel) or without (bottom panel) Rtn4a-GFP. Note that Rtn4a-GFP tubules are less branched (top first panel) and exclude KDEL from the periphery (top second panel). (B) Zoom from A shows overlay of t=0sec (red) with t=60sec (green) to show ER movement over time “dynamics”. (C) Graph of average Pearson’s correlation coefficient shows greater co-localization and therefore fewer dynamic ER movements for Rtn4a expressing cells (control n=22 cells, Rtn4a n=20 cells, ***, p<0.001, two-tailed test). (D) Graph showing no significant difference in endosome diameter between control and Rtn4a expressing cells (control n=216 endosomes, Rtn4a n=215 endosomes). (E) Graph showing no significant difference in endosome bud length between control and Rtn4a expressing cells (control n=257 buds in 22 cells, Rtn4a n=257 buds in 20 cells). (F) Graph showing a significant (**, p<0.01, two-tailed test) decrease in the percent of endosome buds from E that undergo productive fission in Rtn4a expressing cells. Scale bars = 5μm in A, and 1μm B and G. Error bars represent SEM.

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