Table 3.

 Examination of clinical specimens derived from field cases of H1N1v‐infected pigs using RRT PCR assays

Country of origin	Clinical Specimen (numbers)	Ct values of RRT PCR		Confirmatory criterion for H1N1v infection in herd
		“Perfect match” M gene RRT PCR	H1‐118 RRT PCR
Singapore (Quarantine)	Nasal swab (1)	25·84	25·50	Sequencing and BLAST confirmation as H1N1v for both clinical specimens and egg‐isolated virus
	Tracheal swab (1)	26·05	25·99
N Ireland (a)	Lung (4)	21·48	22·34	Sequencing and BLAST confirmation as H1N1v for all four clinical specimens
		20·84	20·89
		28·29	29·05
		23·99	24·33
N Ireland (b)	Lung (1)	30·26	30·20	Sequencing and BLAST confirmation as H1N1v for clinical specimen and egg‐isolated virus
Norway	Nasal swabs (12)	23·04	23·14	Sequencing and BLAST confirmation as H1N1v for two swabs
		20·64	20·74
		26·67	26·40
		26·08	26·02
		No Ct	No Ct
		34·14	34·66
		27·97	28·15
		32·23	32·48
		28·84	28·45
		28·71	29·00
		24·15	23·80
		29·83	29·55
Ireland	Lung (4)	23·71	24·24	Sequencing and BLAST confirmation as H1N1v for two lung specimens
		22·21	22·69
		26·77	27·22
		24·98	25·31
N Ireland (c)	Lung (2)	37·50	37·93	Epidemiologically linked to other cases of H1N1v in swine in N Ireland & Ireland
		33·17	32·84
Iceland	Nasal swabs (10)	30·64	29·92	Sequencing and BLAST confirmation as H1N1v for swab
		27·62	28·02
		27·60	27·87
		29·00	29·04
		25·98	26·18
		24·09	24·20
		26·41	26·40	Sequencing and BLAST confirmation as H1N1v for swab
		28·17	28·25
		23·87	24·45
		23·23	23·38
England (a)	Lung tonsil & trachea pools (3)	28·02	27·77	Sequencing and BLAST confirmation as H1N1v for this pooled tissue
		23·95	23·86
		30·41	29·86
England (b)	Lung tonsil & trachea pool (1)	21·95	22·30	Sequencing and BLAST confirmation as H1N1v for clinical specimen and egg‐isolated virus

Ct values are shown for both generic “Perfect match” M gene RRT PCR and the specific “H1‐118” RRT PCR assays.