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. 2007 Jan 19;1(1):19–25. doi: 10.1111/j.1750-2659.2006.00005.x

Figure 1.

Figure 1

 Schematic diagram of the launch vector pBID4. The vector contains the 35S promoter of cauliflower mosaic virus (a DNA plant virus) that drives initial transcription of the recombinant viral genome following introduction into plants, and the nos terminator, the transcriptional terminator of Agrobacterium nopaline synthase. The tobacco mosaic virus genome contains genes for virus replication (126/183k) and cell‐to‐cell movement (MP), and the cloned target gene, inserted into a unique cloning site and under the transcriptional control of the coat protein subgenomic mRNA promoter (arrow). Left and right border sequences (LB and RB) of the Agrobacterium binary plasmid delimit the region of the launch vector that is transferred into plant cells following infiltration of plants with the recombinant Agrobacterium.