Skip to main content
. 2009 Jun 15;3(4):177–182. doi: 10.1111/j.1750-2659.2009.00087.x

Figure 1.

Figure 1

 Lipopeptide vaccines induce potent IFN‐γ producing CD8+ T cells. Groups of BALB/c mice (n = 3 in A, n = 5 in B) were immunized i.n. with 20 nmol of lipopeptide in 50 μl of PBS under Penthrane anaesthesia. (A) Kinetics of lipopeptide‐induced CD8+ T cells. At each indicated time point, single cell suspensions of the spleens, MLN and lungs were prepared and stained with anti‐CD8 and anti‐IFN‐γ antibodies then analysed by flow cytometry. Data is presented as the total number of CD8+ T cells producing IFN‐γ in response to the CTL epitope peptide minus the number detected in the absence of peptide stimulation. Mean values and standard deviation are shown. (B) Recall of specific vaccine‐induced CD8+ T‐cells responses by virus. Four weeks post lipopeptide vaccination or inoculation with PBS, mice were challenged with 500 pfu of PR8 virus i.n. Single cell suspensions from organs analysed by flow cytometry as in (A). Each individual symbol represents an individual mouse with the lines indicating the mean value.