Table 1.

 Signs of infection in mice challenged with A/chicken/Italy/13474/99 (H7N1) virus

Vaccine	No. of mice (n = 20)
	Died	Weight loss >10%	Virus recovery	Clinical symptoms	Disease free
12 μg	1**	9	15	2	4
12 μg + AlOOH	0**	4	12	0	7
20 μg	0**	8	16	0	3
20 μg + AlOOH	1**	3	12	2	8
None	17	20	20	20	0

Following viral challenge, animals were weighed and observed for death and clinical symptoms (ruffled fur, neurological signs, respiratory and ocular symptoms) for 14 days and nasal washings were collected for 6 days to monitor viral shedding. The table shows the number of mice exhibiting signs of infection on any of the observation days. Disease‐free animals are defined as mice that did not show any of the following: weight loss, virus recovery and clinical symptoms. The statistical analysis for the clinical scores was based on the data collected from 1 to 14 days post‐challenge. As the clinical score data were not normally distributed, a non‐parametric analysis of variance and the Wilcoxon rank test was used for performing pair‐wise comparisons.

**Significantly lower than the control (none) group (P < 0·001).