Table 1. Selected Primers for PCR and gene sequencing.

Primers	Sequence	Annealing temperature	Base pair
BRAF F exon 15	TCATAATGCTTGCTCTGATAGGA	55.5°C	185
BRAF R exon 15	GGCCAAAAATTTAATCAGTGGA
K-RAS F2 exon 2	GTTTGTATTAAAAGGTACGGTGGA	58°C	270
K-RAS R2 exon 2	ATCAAAGAATGGTCCTGCAC
K-RAS F2 exon 3	CCAGACTGTGTTTCTCCCTTC	59°C	288
K-RAS R2 exon 3	TATGCATGGCATTAGCAAAGACTC
PIK3CA F exon 9	TCCAGTCACTGTGCTGCTTC	56.8°C	487
PIK3CA R exon 9	GCAAGGGAAAAGGGAGTCTT
PIK3CA F1-nested exon 9	TTGCTTTTTCTGTAAATCATCTGTG	55.5°C	270
PIK3CA R2-nested exon 9	GCCAAATTCAGTTATTTTTTCTGT
PIK3CA F exon 20	TGACATTTGAGCAAAGACCTG	59.4°C	445
PIK3CA R exon 20	GGATTGTGCAATTCCTATGC
PIK3CA F-hemi exon 20	AGGTTTCAGGAGATGTGTTAC	59.4°C	372
PIK3CA R exon 20	GGATTGTGCAATTCCTATGC
EGFR F exon 18	GCTTGCAAGGACTCTGGGCT	62°C	360
EGFR R exon 18	CCAAACACTCAGTGAAACAAAGAG
EGFR F exon 19	GTGCATCGCTGGTAACATCCA	55°C	306
EGFR R exon 19	CATTTAGGATGTGGAGATGAGC
EGFR F exon 20	GAAACTCAAGATCGCATTCATGC	60°C	379
EGFR R exon 20	GCAAACTCTTGCTATCCCAGGAG
EGFR F exon 21	CTAACGTTCGCCAGCCATAAGTCC	57°C	370
EGFR R exon 21	GCTCACCCAGAATGTCTGGA
AKT1 F exon 2	AGGCACATCTGTCCTGGCAC	61°C	263
AKT1 R exon 2	AAATCTGAATCCCGAGAGGCC