Figure 5.

As₄S₄ combined with cisplatin or celecoxib alters the expression of several apoptosis-related proteins.

Notes: (A) Anti-COX2, anti-cyclin D1 Western blot analysis of HCT116 cells treated with 5 μM As₄S₄ for 0, 2, 6, 12, and 24 hours. (B) Relative quantitation and statistical analysis of protein expression in the Western blot in (A). (C) Western blot analysis of AGS cells treated with 1.5 μM As₄S₄, and/or 7.5 μg/mL cisplatin and/or 20 μM celecoxib for 24 hours. Antibodies used in the blotting are indicated. (D) Relative quantitation and statistical analysis of protein expression in the Western blot in (C). (E) Western blot analysis of AGS cells treated with 1.5 μM As₄S₄, and/or 7.5 μg/mL cisplatin for 24 hours. Antibodies used in the blotting are indicated. β-actin was used as a loading control. (F) Relative quantitation and statistical analysis of protein expression in the Western blot in (E). ^*P<0.05, ^**P<0.01, ^***P<0.001.

Abbreviations: As₄S₄, arsenic sulfide; h, hours; p-p38, phosphor-p38; c-caspase 3, cleaved caspase 3.