Fig. 5.

Expression of genes associated with DNA damage induced by overexpression of ΔDNMT3B4-del. (A) Immunohistochemistry for proteins as indicated in a series of sections from lungs of wild-type and transgenic animals. (B) Scheme of major components in inducible expression of ΔDNMT3B4-del in lentiviral expression system. (C) EGFP expression driven by tet-op promoter through IRES fragment in the presence of 1 μg/ml Dox, an indirect proof of ΔDNMT3B4-del expression. (D) Cell cycle distributions measured in the immortalized normal bronchial epithelial (HBE4) cells infected with lentivirus hosting the inducible expression vectors of ΔDNMT3B4-del before and after 48 h treatment with 1 μg/ml Dox. (E) Levels of aneuploidy and DNA damage associated proteins in HBE4 cells infected with lentiviral particles before and after 48 h treatment with Dox.