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. 2015 Sep 7;2(10):1340–1350. doi: 10.1016/j.ebiom.2015.09.002

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© 2015 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Supplementary Figure 2 — Expression of ΔDNMT3B isoforms and the cellular localization of ΔDNMT3B4. (A), Western blot analysis using antibody against V5 tag in cells transfected with various ΔDNMT3B plasmid constructs with V5 tag sequence. Cells transfected with pcDNA6/V5–His-ΔDNMT3B4 (Lanes 1 and 2); lipofectamine only (Lane 3); pcDNA6/V5–His control vector (Lane 4); pcDNA6/V5–His-ΔDNMT3B4-del (Lane 5); pcDNA6/V5–His-ΔDNMT3B3-del (Lane 6); pcDNA6/V5–His-ΔDNMT3B2-del (Lane 7) and pcDNA6/V5–His-ΔDNMT3B1-del (Lane 8). (B), HEK293 cells were transfected with ΔDNMT3B4-del-GFP fusion protein construct. The expression and cellular location of ΔDNMT3B4-del was detected under fluorescence microscope.