Fig. 2.

H₂O₂ accumulation and scavenging by mouse brain mitochondira oxidizing succinate. A, typical experiment to estimate ROS accumulation in the medium containing mitochondira oxidizing succinate (see “Methods”). Black circles indicate the data points (the H₂O₂ amount that was accumulated in the mitochondria suspension at the indicated time) used to construct the graphs presented on panels B and C. B, the H₂O₂ accumulation dynamics at different concentrations of mitochondria protein. Dashed lines indicate the dynamics of H₂O₂ accumulation calculated by the Eq. 3 (see the text). C, the data from B after normalizing the amount of H₂O₂ by the protein content in the assay. D, typical H₂O₂ scavenging dynamics with succinate-oxidizing mitochondria. White triangles and squares indicate the amount of H₂O₂ remaining in the assay medium at the indicated time points. The points at “0 sec” were obtained by adding H₂O₂ to the assay medium in the absence of mitochondria