Fig. 2.

Deletions or mutations in C-terminal ends of TaCIPKs dramatically changes its ability to interact with TaCBLs. a series deletions in C-terminal ends of TaCIPK11 leads to changing interacting patterns with TaCBLs. The boxes indicate the PKC and NAF domains, and numbers beside the lines represent the beginning and the ending positions of TaCIPK11 protein fragments. b, C-terminal ends alignment between TaCIPK17-A and TaCIPK17-B. Residues with grey background indicates mutant amino acid sites. c, interactions of TaCIPK17-A and TaCIPK17-B with TaCBLs. Yeast was monitored on the selection medium and indicated as growth (+) and no growth (−). d, Schematic representation of the “concave-convex” model