Fig. 3.

Expression profile analyses of TaCBL and TaCIPK in wheat tissues with microarray data a, b and c) and RT-PCR (d and e). a gene expression analyses in wheat whole life cycle using public microarray data. b, gene expression analyses during seeds germination process after immersed with water. c, comparative transcriptomic analyses between dormant and after-ripened seeds in both imbibed and ABA application states. The value (b and c) represents relative transcript levels compared to control (before immersion; 0 h). The up panels in a b and c represent log2 transformed values. d, gene expression analyses during wheat development using RT-PCR method. “S” and “F” represent seedlings and flowering stages, respectively. e, the induced genes in seeds germination (b) were confirmed by RT-PCR