Table 2.
Interactions of TaCIPKs with TaCBLs in yeast two-hybrid assay
| TaCBL1-BD | TaCBL2-BD | TaCBL3-BD | TaCBL4-BD | TaCBL6-BD | TaCBL7-BD | TaCBL9-BD | |
|---|---|---|---|---|---|---|---|
| TaCIPK3-AD | + | + | + | + | - | - | - |
| TaCIPK5-AD | + | + | - | + | + | + | - |
| TaCIPK7-AD | - | - | - | - | - | - | - |
| TaCIPK8-AD | - | - | + | - | - | - | - |
| TaCIPK10-AD | - | - | - | - | - | - | - |
| TaCIPK11-AD | - | + | + | - | + | - | + |
| TaCIPK14-AD | + | + | + | + | + | + | - |
| TaCIPK15-AD | + * | + | + | +* | +* | + | - |
| TaCIPK17-AD | - | + | + | - | - | - | - |
| TaCIPK19-AD | - | - | - | - | - | - | - |
| TaCIPK21-AD | - | + | + | - | + | + | - |
| TaCIPK22-AD | - | - | - | - | - | + | - |
| TaCIPK24-AD | - | - | - | - | - | - | - |
| TaCIPK25-AD | + | + | - | - | - | - | - |
| TaCIPK26-AD | - | + | + | + | - | + | - |
| TaCIPK27-AD | - | + | +* | - | + | -* | - |
| TaCIPK28-AD | - | - | - | - | - | - | - |
| TaCIPK29-AD | - | - | - | - | - | - | - |
| TaCIPK31-AD | - | +* | + | + | - | + | +* |
| TaCIPK32-AD | - | + | + | - | - | - | - |
The interaction analyses of wheat TaCBL and TaCIPK proteins were performed by Y2H method. TaCIPKs and TaCBLs were respectively cloned to PGAD and PGBK Vector, and then co-transformed into Y187 strains. The transformants containing the target plasmid combinations were grown on selection medium (TDO: SD/–Trp/–Leu/–His/+10 mM 3AT) and indicated as growth (+, interaction) and no growth (−, no interaction). “*” represents the interactions were verified by BiFC assays