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. 2015 Nov 4;16:367. doi: 10.1186/s12859-015-0764-0

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© Müller et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Dependence of reconstruction quality on the refractive index variation. a The refractive index values of the cell phantom range from 1.334 to 1.455 (cytoplasm), 1.435 (nucleus), and 1.543 (nucleolus) in a linear fashion. The approximate range of the refractive index for biological cells is marked in green. The refractive index of the medium is 1.333. b Normalized root mean square (RMS) error in dependence of the refractive index values shown in (a). c Normalized total variation (TV) error in dependence of the refractive index values shown in (a). d, e, f Cross sections of the 3D refractive index reconstruction with the Rytov approximation. The three simulations are labeled in (c). The diameter (2a=17 λ) of the cell phantom and the total number of projections (200) are unchanged for all simulations