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. 2015 Nov 5;11(11):e1005664. doi: 10.1371/journal.pgen.1005664

Fig 2. Rad51p and Rad59p localize to the mitochondrial matrix.

Fig 2

(A) Intact mitochondria, mitoplasts, or Triton X-100 lysed mitochondria were treated with proteinase K and subjected to immunoblot analysis with the indicated antibodies. Por1p, outer membrane protein; Cob, intermembrane space protein; Cit1p, matrix protein. (B) Rad51p physically interacts with mtDNA. DNA samples (3 biological replicates) were immunoprecipitated with antibodies against endogenous Rad51p or V5 epitope tag (mock). Samples were evaluated by qPCR and changes in fold enrichment were compared to the mock IP. Error bar indicates SD. Asterisks indicate significant differences between the Rad51p IP and the mock IP (** = p ≤ 0.01).