Fig 6. Repair of induced mitochondrial DSBs in HR single mutants.

All samples were obtained after 16–18 hours of mtLS-KpnI induction. DNA was extracted from the appropriate strains and digested with AvaII. Samples in lanes labeled P are from pre-induced cultures. Lane I is the t = 0 sample from the mtLS-KpnI-intein^ts expressing strain digested with KpnI and AvaII to demonstrate the migration of the reporter with a DSB in relation to the other COX2 bands. (A) Representative Southern blot of rad51-Δ strains ASY114 (intein^dead) and ASY113 (intein^ts). (B) Representative Southern blot of rad52-Δ strains ASY116 (intein^dead) and ASY115 (intein^ts). (C) Representative Southern blot of rad59-Δ strains ASY118 (intein^dead) and ASY117 (intein^ts). (D) A representative Southern blot of rad51-Δ rad52-Δ strains, ASY120 (intein^dead) and ASY119 (intein^ts).