Fig 2. Analyses and Identification of MiR-29c Promoter.

(a). UCSC genome browser shows location of the miR-29c/29b2 gene at chromosome 1q32. Two primary transcripts are shown with GenBank ID, EU154352 and EU154352. Potential promoter region located 20 kb (region A) or 1Kb (region B) upstream of the gene with 6 or 1 CAGE tags, respectively, were indicated with red boxes. The enhancer (H3K4me1) and promoter (HeK4Me3) markers of histone modifications from ENCODE are also shown. (b). ChIP against RNA Polymerase II (Pol II) revealed enrichment of Pol II at the 20kb-upstream promoter (region A). PCR were performed using two primer sets (primer set 1 and 2) targeting at each promoter. ChIP without any antibody (No Ab) or with an antibody against Mouse IgG was used as a negative control. (c) Luciferase activity was measured in MDA-MB-231 cells transfected with pGL₃-Basic luciferase reporter vectors or miR-29c promoter-luciferase constructs. The means±SD from three experiments are shown.