Table 2. Embryonic lethality induced by constitutively active IKK2 can be prevented by an IκBα superrepressor.
| MyHC-tTA | IKK2-CA | IκBα-3M | Numbers observed | Numbers expected | % observed | % expected |
|---|---|---|---|---|---|---|
| - | - | - | 5 | 9.6 | 6.5 | 12.5 |
| + | - | - | 11 | 9.6 | 14.3 | 12.5 |
| - | + | - | 18 | 9.6 | 23.4 | 12.5 |
| Functionally wild type | 34 | 28.9 | 44.2 | 37.5 | ||
| + | + | - | 1 | 9.6 | 1.3 | 12.5 |
| IKK MyHC | 1 | 9.6 | 1.3 | 12.5 | ||
| - | - | + | 12 | 9.6 | 15.6 | 12.5 |
| + | - | + | 11 | 9.6 | 14.3 | 12.5 |
| - | + | + | 12 | 9.6 | 15.6 | 12.5 |
| Functionally NF-κB-inhibited | 35 | 28.9 | 45.5 | 37.5 | ||
| + | + | + | 7 | 9.6 | 9.1 | 12.5 |
| IKK MyHC / IκBα-3M | 7 | 9.6 | 9.1 | 12.5 | ||
| Total | 77 | 77 | 100 | 100 | ||
The table shows the absolute number and percentage of animals born with the indicated actual and functional genotype (observed), and the corresponding expected number and percentage (expected). Only one IKKMyHC animal was identified, whereas seven IKKMyHC / IκBα-3M mice could be detected, indicating a rescue of the lethality by the IκBα-3M transgene.