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. 2015 Nov 5;10(11):e0142039. doi: 10.1371/journal.pone.0142039

Fig 2. Effect of orchidectomy and the DHA-supplemented diet on the production of endothelial NO and superoxide anion from rat aorta.

Fig 2

Confocal micrographs showing in situ detection of NO (A) or superoxide anion (B) in aortic segments from control (C) and orchidectomized (ORX) rats fed with a control diet or with a DHA-supplemented diet (DHA). The sections shown are typical preparations from five rats. Quantitative analysis of fluorescence is also shown. Values are means ± SEMs. Number of animals, n = 5. *P < 0.01 compared with control animals; +P < 0.01 compared with ORX rats control diet.