Table 3. Intra Class Correlation coefficients for all non-diabetes controls (n = 20).

	Observer reliability measure	The manual protocol ICC and 95%CI*	The semi-automated protocol ICC and 95%CI	Comparison of semi-auto to manual P-Value	The fully automated protocol ICC and 95%CI	Comparison of fully auto to manual P-Value	Comparison of fully auto to semi-auto P-Value
CNFL	Inter	0.68(0.35,0.86)	0.73(0.43,0.88)	0.34	0.75(0.25,0.91)	0.38	0.50
	Intra	0.72(0.39,0.88)	0.77 (0.50,0.90)	0.32	0.81(0.59,0.92)	0.16	0.31
CNFD	Inter	0.39(-0.04,0.70)	0.72(0.42,0.88)	0.018	0.52(0.09,0.78)	0.27	0.92
	Intra	0.51(0.10,0.78)	0.69(0.36,0.87)	0.11	0.72(0.39,0.88)	0.084	0.41
CNBD	Inter	0.35(-0.11,0.71)	0.44(0.04,0.73)	0.31	0.50(0.08,0.77)	0.23	0.39
	Intra	0.58(0.21,0.81)	0.58(0.08,0.75)	0.74	0.49(0.09,0.76)	0.70	0.47

ICC: Intra-class correlation coefficient (2, 1). Inter refers to inter-observer reproducibility, intra to intra-observer reproducibility. The manual protocol included manual image selection and manual image analysis. The semi-automated protocol included manual image selection and automated analysis. The fully-automated protocol included automated image selection and automated analysis. CNFL, corneal nerve fibre length. CNFD, corneal nerve fibre density. CNBD, corneal nerve branch density.

* Manual protocol ICC have been presented in a previous publication and are shown here for comparison.