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. 2015 Nov 5;10(11):e0141897. doi: 10.1371/journal.pone.0141897

Fig 4. ·NO-induced down-regulation of topo I protein levels in MCF-7 cells.

Fig 4

(A) Cells were treated for various times with PPNO and cells were collected, lysed and analyzed by Western blots for topo I, (B) Quantification of the resulting topo I protein levels, and (C) Cytotoxicity of CPT (■-■) alone and in the presence of PPNO (100 μM; ●-●, 50 μM; ▲-▲) in MCF-7 cells. Values are mean SEM from at least 3 separate experiments carried out in triplicate (n = 3). *P<0.05, **P<0.01, # # and ***P<0.001, # # # and with respect to concentration-matched samples.