Fig 1. Cell cycle analysis of Colon 38 cells treated by Malformin C and its combinations.

(A) Chemical structure of Malformin C. Malformin C is a member of Malformins, a group of fungal cyclic pentapeptides. Its chemical formula is C₂₃H₃₉O₅N₅S₂ with a molecular weight of 529.7. (B) Cell cycle progression of Colon 38 cells exposed to an increasing concentration of Malformin C for 24 hours. (C) Cell cycle progression of Colon 38 cells exposed to an increasing concentration of Malformin C for 48 hours. (D) The dose-dependent accumulation of G2-M phase Colon 38 cells treated by Malformin C at concentrations of 90 nM, 270 nM and 810 nM. Compared to the control group, the symbol Δ represented P<0.05, while * represented P<0.01. (E) Cell cycle analysis of Colon 38 cells treated with combinations of Malformin C and SN38. All the cells were exposed to respective compounds indicated above the graph for 24 hours and the analysis was done in duplicate. When treated with SN38, no significant changes of cell cycle progression were observed without or with different dosage of Malformin C.